Overview
MEF feeders are prepared weekly to provide a substrate for undifferentiated embryonic stem (ES) cells. Primary MEF cells are thawed, established in culture, treated with mitomycin C to halt their proliferation so they cannot overgrow the ES cultures, and then replated onto dishes convenient for ES cell culture. This protocol can also be used to prepare feeder cells from STO fibroblast cell lines.
Linda C. Samuelson and Joseph M. Metzger
Details
Details of this protocol, Preparation Of Mouse Embryonic Fibroblast (MEF) Feeder Plates (Subscription Required), are located on a web site other than Biocompare Protocols.