Overview
This protocol describes the isolation of fibroblasts from mouse embryos. Mouse embryonic fibroblast (MEF) cells are used as a feeder layer for the culture of mouse embryonic stem (ES) cells to help maintain them as pluripotent stem cells. The inhibition of ES-cell differentiation provided by the MEF feeders appears to be due to their production of leukemia inhibitory factor (LIF). MEF cells are isolated from embryos, established in culture in bulk, and then frozen in convenient aliquots to be thawed for MEF feeder cell preparation.
Linda C. Samuelson and Joseph M. Metzger
Details
Details of this protocol, Isolation And Freezing Of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates (Subscription Required), are located on a web site other than Biocompare Protocols.