Ribocloning: DNA Cloning And Gene Construction Using PCR Primers Terminated With A Ribonucleotide (Subscription Required) Protocol

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Title	Ribocloning: DNA Cloning And Gene Construction Using PCR Primers Terminated With A Ribonucleotide (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4142

Overview

This protocol exploits the discovery that RNase A can efficiently cleave at single rC or rU bases embedded in double-stranded DNA. Entire plasmid vectors are amplified using long, high-fidelity PCR with riboprimers, which carry a single rC residue at their 3' end. By Wayne M. Barnes.

Details

Details of this protocol, Ribocloning: DNA Cloning And Gene Construction Using PCR Primers Terminated With A Ribonucleotide (Subscription Required), are located on a web site other than Biocompare Protocols.