Overview
This method, for the selective amplification of full-length cDNA ends, involves the addition of an adapter during reverse transcription. This method takes advantage of the propensity of Moloney murine leukemia virus reverse transcriptase (MMLV RT) to append two to four cytosines to the 3'-end of newly synthesized cDNA strands. The additional residues are added when the enzyme reaches the 5'-cap structure at the end of the mRNA template (Schmidt and Mueller 1999; Schramm et al. 2000). By Michael A. Frohman.
Details
Details of this protocol, Cap-Switching RACE (Subscription Required), are located on a web site other than Biocompare Protocols.