Overview
To generate "5'-end" partial cDNA clones using classic RACE, the first-strand products are generated by reverse transcription (primer extension) from a known gene-specific primer (GSP-RT). Then, a poly(A) tail is appended using terminal deoxynucleotidyltransferase (Tdt) and dATP. By Michael A. Frohman.
Details
Details of this protocol, 5'-End CDNA Amplification Using Classic RACE (Subscription Required), are located on a web site other than Biocompare Protocols.