Overview
The following protocol uses an autoregulatory system in which the transcriptional trans-activator tTA drives its own expression and that of a target gene. The first stage of the procedure describes how to generate stable lines of NIH-3T3 cells that express either tTA alone or tTA and the tetracycline-regulated target gene.
Details
Details of this protocol, Tetracycline As Regulator Of Inducible Gene Expression - Stage 1: Stable Transfection Of Fibroblasts With PTet-TTAk (Subscription Required), are located on a web site other than Biocompare Protocols.