Tetracycline As Regulator Of Inducible Gene Expression - Stage 2: Stable Transfection Of Inducible TTA-Expressing NIH-3T3 Cells With Tetracycline-Regulated Target Genes (Subscription Required) Protocol

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Title	Tetracycline As Regulator Of Inducible Gene Expression - Stage 2: Stable Transfection Of Inducible TTA-Expressing NIH-3T3 Cells With Tetracycline-Regulated Target Genes (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot3957

Overview

This stage of the procedure describes the transfection with target genes of cell lines already expressing inducible tTA. In this example, the target genes are transfected on a plasmid that carries puromycin resistance as a selectable marker.

Details

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