Overview
This protocol describes the use of PCR to screen for bacteria that carry recombinant plasmids. The PCR can be carried out using the same primers as for amplification of the cloned insert. To determine the orientation of the insert, a third, insert-specific primer that is asymmetrically distanced from the clonal insertion site can be used. By Gina L. Costa and Michael P. Weiner.
Details
Details of this protocol, Colony PCR (Subscription Required), are located on a web site other than Biocompare Protocols.