Overview
This protocol describes the purification, quantification, and subsequent sequencing of amplified DNA fragments using PCR. Excess nucleotides are removed from the initial PCR products using spun columns, and the products are quantified using fluorometry. The fragments are sequenced using a cycle sequencing kit and, after purification, the sequencing reactions are analyzed on an automated DNA sequencer.
Details
Details of this protocol, Nonradioactive Cycle Sequencing Of PCR-Amplified DNA (Subscription Required), are located on a web site other than Biocompare Protocols.