Overview
In this protocol, double-stranded DNA probes, labeled in each strand, are produced in conventional PCRs containing equal concentrations of two primers, a double-stranded DNA template, three unlabeled dNTPs at concentrations exceeding the Km, and one [{alpha}-32P]dNTP at a concentration at or slightly above the Km (2-3 µm) for a thermostable DNA polymerase such as Taq. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Radiolabeling Of DNA Probes By The Polymerase Chain Reaction (Subscription Required), are located on a web site other than Biocompare Protocols.