Overview
Quantitative PCR involves co-amplification of two templates: a constant amount of a preparation containing the desired target sequence and serial dilutions of a reference template that is added in known amounts to a series of amplification reactions. The concentration of the target sequence is determined by simple interpolation into a standard curve. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Quantitative PCR (Subscription Required), are located on a web site other than Biocompare Protocols.