Overview
In this method, sequences cloned in standard bacteriophage or plasmid vectors are amplified in PCRs containing primers targeted to flanking vector sequences. The amplified fragments can be analyzed by gel electrophoresis, DNA sequencing, and/or restriction mapping. Many colonies or plaques can be assayed simultaneously. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Rapid Characterization Of DNAs Cloned In Prokaryotic Vectors (Subscription Required), are located on a web site other than Biocompare Protocols.