Overview
In MOPAC, the amino-terminal and carboxy-terminal sequences of a peptide are used to design two redundant families of oligonucleotides encoding the amino- and carboxy-terminal sequences of the peptide. The primers are used either to amplify a segment of cDNA prepared by RT-PCR from a tissue known to express the protein or to amplify a segment of DNA from an established genomic or cDNA library. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Mixed Oligonucleotide-Primed Amplification Of CDNA (MOPAC) (Subscription Required), are located on a web site other than Biocompare Protocols.