Assessment of Apoptosis and Necrosis by DNA Fragmentation and Morphological Criteria Protocol

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Title Assessment of Apoptosis and Necrosis by DNA Fragmentation and Morphological Criteria
Source Boris Zhivotosky and Sten Orrenius, Karolinska Institute, Stockholm, Sweden

Overview

Apoptotic cells share a number of common features, such as phosphatidylserine (PS) exposure, cell shrinkage, chromatin cleavage, nuclear condensation, and formation of pyknotic bodies of condensed chromatin. Necrotic cells exhibit nuclear swelling, chromatin flocculation, loss of nuclear basophilia, breakdown of cytoplasmic structure and organelle function, and cytolysis by swelling. This unit describes some of the techniques most commonly used to detect cell death.

Details

Details of this protocol, Assessment of Apoptosis and Necrosis by DNA Fragmentation and Morphological Criteria, are located on a web site other than Biocompare Protocols.

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