Imaging Protein Interactions By FRET Microscopy: Cell Preparation For FRET Analysis (Subscription Required) Protocol

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Title	Imaging Protein Interactions By FRET Microscopy: Cell Preparation For FRET Analysis (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4646

Overview

The following protocol describes the preparation of cells for FRET analysis on live and fixed cells. The reagents used have been optimized to minimize the quenching of GFP mutants and fluorescent dyes. By Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens.

Details

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