Overview
Use of the chemiluminescence-producing alkaline phosphatase substrate 3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo-[3.3.1.1(3,7)]decan]-4-yl)phenyl phosphate (AMPPD, also known as adamantyl-1,2-dioxetane phosphate), or its dioxetane relatives (e.g., CSPD, a halogen-substituted derivative of AMPPD) provides a substantial increase in sensitivity over colorimetric substrates and radiochemical methods currently used for the detection of antigen-antibody complexes immobilized on nylon or PVDF membranes. With many antigen-antibody combinations, the method is capable of detecting as little as 1 pg of protein.
Peter Gillespie
Details
Details of this protocol, Western Analysis Using The Chemiluminescent Alkaline Phosphatase Substrate CSPD (Subscription Required), are located on a web site other than Biocompare Protocols.