Overview
This image acquisition protocol is a basic plan for taking a fluorescence lifetime imaging (FLIM) series. FLIM makes live-cell FRET measurements based only on donor fluorescence more feasible, because lifetimes are independent of probe concentration and light path length. The former is not easy to determine in cells, and the latter means that cell shape is not a factor. By Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens.
Details
Details of this protocol, Imaging Protein Interactions By FRET Microscopy: FLIM Measurements (Subscription Required), are located on a web site other than Biocompare Protocols.