Overview
This protocol describes the detection of fluorescence resonance energy transfer (FRET) by measuring the quenching of donor emission alone. As opposed to sensitized emission measurements, photobleaching can be performed with high selectivity of the acceptor because absorption spectra are steep at their red edge, allowing the acceptor to be bleached without excitation of the donor. By Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens.
Details
Details of this protocol, Imaging Protein Interactions By FRET Microscopy: FRET Measurements By Acceptor Photobleaching (Subscription Required), are located on a web site other than Biocompare Protocols.