Overview
This protocol describes a method for measuring fluorescence resonance energy transfer (FRET) by the detection of acceptor-sensitized emission. This approach is useful in situations where donor intensities are low and/or there is contamination with high background (auto) fluorescence in the donor channel. By Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens.
Details
Details of this protocol, Imaging Protein Interactions By FRET Microscopy: FRET Measurements By Sensitized Emission (Subscription Required), are located on a web site other than Biocompare Protocols.