Overview
The following are key elements of a frequency domain fluorescence lifetime imaging system: (1) a standing wave acousto-optic modulator (SW-AOM) that modulates the intensity of the excitation light source at high frequency and (2) a frequency mixing device, such as an image intensifier, to perform phase-sensitive detection of the fluorescence emission. By Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens.
Details
Details of this protocol, An Experimental Setup For Frequency Domain FLIM (Subscription Required), are located on a web site other than Biocompare Protocols.