Overview
Occurrence of FRET leads to a decrease in the intensity of the donor fluorescence that is proportional to the number of donor-tagged molecules that interact with an acceptor-bound molecule. Experimentally, the decrease in intensity can be measured on the specimen by specifically photobleaching the acceptor. By Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens.
Details
Details of this protocol, Measuring FRET By Acceptor Photobleaching (Subscription Required), are located on a web site other than Biocompare Protocols.