Overview
Stably transfected cells, generated in the first two stages of the procedure, are induced for expression of the target gene. After harvesting and lysis, the lysates are analyzed by SDS-PAGE and immunoblotting. For further details on expression using this system, please see http://www.clontech.com/tet/Refs/index.html.
Details
Details of this protocol, Tetracycline As Regulator Of Inducible Gene Expression - Stage 3:Analysis Of Protein Expression In Transfected Cells (Subscription Required), are located on a web site other than Biocompare Protocols.