Overview
The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. By Angelika Görg, Oliver Drews and Walter Weiss.
Details
Details of this protocol, Extraction And Solubilization Of Total Protein From Microorganisms (Subscription Required), are located on a web site other than Biocompare Protocols.