Genome-Wide Analysis Of Protein-Protein Interactions Using A Two-Hybrid Array: Cloning By Homologous Recombination And High-Throughput Transformation - Subscription Required Protocol

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Title	Genome-Wide Analysis Of Protein-Protein Interactions Using A Two-Hybrid Array: Cloning By Homologous Recombination And High-Throughput Transformation - Subscription Required
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4562

Overview

This procedure capitalizes on the asexual/sexual reproductive cycle of yeast cells. Laboratory yeast strains can be maintained indefinitely by asexual reproduction as one of two haploid mating types, MATa or MAT{alpha}. By Tony R. Hazbun and John P. Miller.

Details

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