Overview
This protocol describes the first step in constructing an array: amplification of the predicted ORFs that are to be included in the array. Gene-specific primers containing vector-specific flanking sequences that facilitate recombinational cloning are used to amplify each ORF. A secondary amplification can be used to extend the length of the homologous vector sequence flanking the ORF. By Tony R. Hazbun and John P. Miller.
Details
Details of this protocol, Genome-Wide Analysis Of Protein-Protein Interactions Using A Two-Hybrid Array: Amplification Of ORFs - Subscription Required, are located on a web site other than Biocompare Protocols.