Overview
A cDNA library constructed in a plasmid expression vector of the pUC, pUR, or pEX series is plated on agar medium and then replicated onto filters, which are transferred to plates containing IPTG. After 2-4 hours of induction, the colonies are lysed with chloroform and then screened with appropriate antibodies. The antigen-antibody complexes are detected by standard radiochemical, chromogenic, or chemiluminescent methods. By Joseph Sambrook and David W. Russell.
Details
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