Detection Of RNAi-Induced Protein Knockdown In Mammalian Cells By Western Blotting-SUBSCRIPTION REQUIRED Protocol

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Title	Detection Of RNAi-Induced Protein Knockdown In Mammalian Cells By Western Blotting-SUBSCRIPTION REQUIRED
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4345

Overview

This protocol describes the use of western blotting to analyze the RNAi-induced depletion of target protein from mammalian cells. Immunofluorescence can be used as an alternative (see Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells).

Details

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