Identifying DNA-Binding Proteins In Bacteriophage {lambda} Expression Libraries - Subscription Required Protocol

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Protocol Information

Title Identifying DNA-Binding Proteins In Bacteriophage {lambda} Expression Libraries - Subscription Required
Source CSH Protocols; 2006; doi:10.1101/pdb.prot3785

Overview

This protocol describes how to identify cloned cDNAs encoding proteins that bind to specific DNA sequences. The methods used are very similar to those used for immunological screening of expression libraries except that the nitrocellulose filters carrying immobilized proteins are screened with 32P-labeled double-stranded DNA rather than with antibodies. By Joseph Sambrook and David W. Russell.

Details

Details of this protocol, Identifying DNA-Binding Proteins In Bacteriophage {lambda} Expression Libraries - Subscription Required, are located on a web site other than Biocompare Protocols.

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