Overview
This protocol describes a method for performing isoelectric fractionation of a maize (Zea mays) embryo sample using a multicompartment electrolyzer (MCE). This prefractionation of proteins having pIs within a certain pH interval is essential for allowing high loads of protein to be resolved on narrow and ultra-narrow immobilized pH gradients used in two dimensional (2D) electrophoresis. The isoelectric membranes in the MCE act like isoelectric traps capturing all the protein species having pIs encompassing the pI value of each pair of membranes delimiting a given chamber. By Ben R. Herbert et al.
Details
Details of this protocol, Fractionation Of Maize Embryo Proteins For Two-Dimensional Gel Electrophoresis Using A Multicompartment Electrolyzer - Subscription Required, are located on a web site other than Biocompare Protocols.