Overview
In this protocol, DNase I is used to fragment a radiolabeled target DNA in the presence and absence of a nuclear extract. A "footprint" is generated when a protein binds to the target and protects a specific segment of DNA from the nucleolytic activity of DNase I. By comparing the electrophoretic mobility of the DNase I cleavage products to those of a sequence ladder derived from the same DNA fragment, the position(s) of the DNA sequences recognized by DNA-binding proteins can be determined. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Mapping Protein-Binding Sites On DNA By DNase I Footprinting - Subscription Required, are located on a web site other than Biocompare Protocols.