Overview
This protocol describes RT-PCR as a method to monitor gene expression by mRNA abundance in RNAi-treated Drosophila embryos. Primers for PCR amplification should have ~50% GC content, be 16-18 nucleotides long, and have a melting temperature of ~60ºC. They should be spaced apart to generate a PCR product 100-200 bp in length. Use the Primer3 program (MIT Genome Center Web Site) for choosing primers. By Richard W. Carthew.
Details
Details of this protocol, Semiquantitative RT-PCR For Phenotype Analysis Of RNAi-Treated Drosophila Embryos, are located on a web site other than Biocompare Protocols.