Synthesis Of DsRNA For RNAi In Drosophila: PCR Template Method Protocol

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Protocol Information

Title Synthesis Of DsRNA For RNAi In Drosophila: PCR Template Method
Source CSH Protocols; 2006; doi:10.1101/pdb.prot4514

Overview

This protocol describes how to prepare double-stranded RNA (dsRNA) for RNA interference in Drosophila by simultaneous synthesis of both RNA strands from a PCR fragment which contains a T7 promoter on each end. dsRNA molecules with a length of 500-800 bp seem to be most active. By Richard W. Carthew.

Details

Details of this protocol, Synthesis Of DsRNA For RNAi In Drosophila: PCR Template Method, are located on a web site other than Biocompare Protocols.

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