RNA Isolation And RT-PCR From DsRNA-Treated Mouse Oocytes And Early Embryos - Subscription Required Protocol

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Title	RNA Isolation And RT-PCR From DsRNA-Treated Mouse Oocytes And Early Embryos - Subscription Required
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4513

Overview

This protocol describes the use of RT-PCR to confirm knockdown of a gene of interest in RNAi-treated mouse oocytes and early embryos. All of the solutions used during RNA isolation and reverse transcription must be RNase-free. By Paula Stein and Petr Svoboda.

Details

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