Overview
This protocol describes a method to stain nerve fibers in tissue slices of avian embryos using an antibody against the 160-kD subunit of neurofilaments. This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos. The protocol is suitable for older embryos after approximately stage 33 and regions that are not accessible by whole-mount analysis. By Esther T. Stoeckli.
Details
Details of this protocol, Staining Of Tissue Slices For Analysis Of Axonal Pathfinding In DsRNA-Treated Avian Embryos (Subscription Required), are located on a web site other than Biocompare Protocols.