Overview
This protocol describes a method for freezing and thawing ES cells using cryovials. It is important to freeze ES cell stocks as soon as possible to reduce the time that they are in culture. A careful record should be kept of the number of times cells are passaged and the location of the cryovials. By Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer.
Details
Details of this protocol, Freezing And Thawing Of Embryonic Stem (ES) Cells Using Cryovials - Subscription Required, are located on a web site other than Biocompare Protocols.