Overview
This protocol describes passage of ES cells. They should be split at 1:3 to 1:7 every 2-3 days depending on their growth rate when they reach 70% confluency. They should never be allowed to grow past 90% confluency, but rather they should form tightly packed colonies not touching each other. By Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer.
Details
Details of this protocol, Passage Of Embryonic Stem (ES) Cells - Subscription Required, are located on a web site other than Biocompare Protocols.