Overview
This protocol describes the preparation of feeder cell layers from MEF cells or from the STO mouse fibroblast cell line. The cells are rendered mitotically inactive by treatment with mitomycin C. The feeder layers can then be used to maintain embryonic stem (ES) cells in the undifferentiated state. By Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer.
Details
Details of this protocol, Preparing Feeder Cell Layers From STO Or Mouse Embryo Fibroblast (MEF) Cells: Treatment With Mitomycin C - Subscription Required, are located on a web site other than Biocompare Protocols.