Overview
This protocol describes the culture of embryonic stem (ES) cells using mitotically inactivating primary mouse embryonic fibroblast (MEF) cells as a feeder layer (preparation described in Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates). The ES culture medium is supplemented with recombinant leukemia inhibitory factor (LIF) to help maintain the cells as pluripotent stem cells. By Linda C. Samuelson and Joseph M. Metzger.
Details
Details of this protocol, Propagation Of Pluripotent Mouse Embryonic Stem (ES) Cells (Subscription Required), are located on a web site other than Biocompare Protocols.