Overview
This protocol describes the direct detection of RNA on DNA microarrays using Hybrid Capture (HC) technology and the HC ExpressArray Kit developed by Diagene. The kit uses a proprietary antibody that binds specifically to RNA:DNA hybrids and a second, fluorescently labeled, antibody that detects the primary antibody. Total RNA is applied directly to a glass-spotted DNA microarray, and stable RNA:DNA hybrids are visualized via a Cy3-labeled secondary antibody. Because the primary antibodies recognize only perfectly matched heteroduplex regions, the strength of the signal generated is proportional to the length of the hybrid formed.
Details
Details of this protocol, Hybridization And Detection Using The HC ExpressArray Kit-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.