Overview
An expression library constructed in a bacteriophage {lambda} vector is plated on an appropriate E. coli strain in the absence of isopropylthio-ß-D-galactoside (IPTG). After 2-4 hours, the plates are moved to 37°C (to stabilize any fusion proteins that are temperature sensitive), and filters impregnated with IPTG are laid on top of the developing plaques. After incubation for a further 2-4 hours, the filters, which contain imprints of the phage-encoded proteins, are removed and probed with antibody. The plates are stored at 4°C until the results of immunological screening are available.
Details
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