Overview
Transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using the submerged method is achieved by placing the gel next to a piece of nitrocellulose filter, submerging this sandwich in a large volume of transfer buffer in a transfer tank, and running current from one side of the transfer tank to another. The proteins are then eluted by transferring them from the gel onto the filter. If carefully assembled, this is an effective method to transfer many proteins to a membrane.
Details
Details of this protocol, Immunoblotting: Submerged Electrophoretic Transfer Of Proteins From Gels To Membranes-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.