Overview
The transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using a semi-dry method is achieved by placing the gel next to a piece of nitrocellulose filter. This sandwich is placed directly between two plate electrodes, and the proteins are then transferred from the gel onto the filter. Good contact between the electrodes, gel, and filter is maintained by using several sheets of pre-wetted glass-fiber filter paper on both the cathode and anode sides. A simple buffer system using diluted Tris/glycine SDS-polyacrylamide gel running buffer is used.
Details
Details of this protocol, Immunoblotting: Semi-Dry Electrophoretic Transfer Of Proteins From Gels To Membranes-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.