Overview
This protocol describes the covalent coupling of antibodies to biotin. Biotin groups bind with extremely high affinity to streptavidin and avidin, both of which are available commercially coupled with enzymes, fluorescent dyes, or iodine. A biotinylated primary antibody, therefore, can be detected with any of a wide variety of different labels. The biotinylation reaction is simple and mild, and rarely inactivates the antibody. Biotinylated antibodies can be stored for years without loss of activity.
Details
Details of this protocol, Labeling Antibodies With Biotin-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.