Overview
Direct labeling of purified antibodies is the method of choice when simultaneously visualizing two or more antibodies of the same species, class, or subclass. This allows the localization of multiple antigens to be compared in the same cell, tissue, or sample. Labeled primary antibodies are also useful for improving background-to-readout ratios, and they can be essential for immunoassays in which good quantification is needed. Fluorescent labels are necessary for cell sorting and high-resolution immunostaining, and they are also an excellent choice for determining precise subcellular localization.
Details
Details of this protocol, Labeling Antibodies With Fluorochromes-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.