Overview
It is possible that some cell lines have lost the ability to perform RNAi or that cells derived from certain tissues do not support RNAi. This reporter assay, for RNAi in mammalian cells, can be used to establish whether the cells under study are susceptible to RNAi. By Thomas Tuschl.
Details
Details of this protocol, Cotransfection Of Luciferase Reporter Plasmids With SiRNA Duplexes - Subscription Required, are located on a web site other than Biocompare Protocols.