Overview
Protein immunoprecipitation can be a useful preparative step for immunoblotting. For very rare proteins, the protein of interest can be purified and concentrated by standard immunoprecipitation techniques before immunoblotting. In addition, protein-protein interactions can be tested with an immunoprecipitating antibody that is specific for one protein of a complex and an immunoblotting antibody that is specific for a second member of the complex.
Ed Harlow and David Lane
Details
Details of this protocol, Immunoblotting: Preparing Immunoprecipitated Proteins-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.