Overview
For immunoblotting experiments, it is often important to compare the total amount of an antigen from many different sources or to learn if a particular source has the antigen under study. In the approach described here, tissue cultures, bacteria, yeast cells, tissues, and other sources of antigens are disrupted directly in an electrophoresis sample.
Ed Harlow and David Lane
Details
Details of this protocol, Immunoblotting: Preparing Cell Lysates-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.