Overview
Fluorescence-linked detection is best used for high-resolution studies. In addition, some fluorochromes have emission spectra that do not overlap, so two fluorochromes can be observed on the same sample. This allows the study of two different antigens in the same specimen even when they have identical subcellular distributions. Flurorescein, rhodamine, and Texas Red are the most commonly used fluorochromes.
Ed Harlow and David Lane
Details
Details of this protocol, Indirect Detection Using Fluorochrome-Labeled Reagents-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.