Overview
Problems with low yield or poor quality can sometimes be overcome by transferring the P1 or PAC recombinant into a strain of E. coli that does not express Cre recombinase. This protocol for transfer of P1 clones by electroporation was supplied by Ray MacDonald (University of Texas Southwestern Medical Center, Dallas).
Details
Details of this protocol, Transferring Bacteriophage P1 Clones Between E. Coli Hosts, are located on a web site other than Biocompare Protocols.