Assay For ß-Galactosidase In Extracts Of Mammalian Cells - Subscription Required Protocol

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Title Assay For ß-Galactosidase In Extracts Of Mammalian Cells - Subscription Required
Source CSH Protocols; 2006; doi:10.1101/pdb.prot3952

Overview

The assay for ß-galactosidase relies on the ability of the enzyme to catalyze the hydrolysis of ONPG (o-nitrophenyl-ß-D- galactopyranoside) to free o-nitrophenol, which absorbs light at 420 nm. By Joseph Sambrook and David W. Russell

Details

Details of this protocol, Assay For ß-Galactosidase In Extracts Of Mammalian Cells - Subscription Required, are located on a web site other than Biocompare Protocols.

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